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KMID : 0380020100250040371
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Korean Journal of Biotechnology and Bioengineering
2010 Volume.25 No. 4 p.371 ~ p.378
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Optimization of Lipid Extraction from Scenedesmus sp. Using Taguchi Approach
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Kim Na-Young
Oh Sung-Ho
Choi Woon-Yong
Lee Hyeon-Yong
Lee Shin-Young
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Abstract
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For the biodiesel fuel production from microalgae, the lipid from wet and dry samples of green algae Scenedesmus sp. was extracted by using various solvents and pre-treatment methods. Extraction yield of the lyophilized sample was better than that of dry sample. Chloroform/methanol (2：1, v/v) and ultrasonication or homogenization method were also selected as the most effective solvent and pre-treatment methods for lipid extraction, respectively. Under these constraint conditions, optimization experiment of lipid extraction was investigated by Taguchi approach using orthogonal matrix L9 (34) method. The optimum extraction conditions of lipid extraction was obtained at pre-treatment of homogenization, extraction time of 5 hour, temperature of 35°C, and solvent ratio of 1：20 (w/v). Yield of extraction at optimized condition was 20.55% and it was 96% of total lipid content (21.38%) of Scenedesmus sp.
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KEYWORD
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Scenedesmus sp. lipid extraction, optimization, Taguchi method
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